I've added 35ml to the estimated 35l in the tank, if that's OK I'll dose the next 25l with 35ml too at Jim's ratio of 1.5:1000.
What is the effect of overdoing it? Knocking? I'm bringing 25l of bio with me to dilute the tank with just in case, though at 1:1000 I gather I'm unlikely to see any issues. I think if anything my fuel pump is slightly retarded.